Level: III
Semester: Year-Long
No. of credits: 3
Prerequisites: BIOL 2164

 

Course Description

This course provides necessary practical skills on recombinant DNA technology and molecular biology and biotechnology techniques. This course will be taught through lab sessions, lab discussions/lectures. Course will be assessed for 100% course work. Lab experiments and lectures will comprehensively cover the experiments and methods involved in gene cloning, necessary instrumentation and Preparation of reagents; Extraction of DNA and RNA; Restriction digestion of plasmid and genomic DNA and fragment analysis; Extraction of plant proteins and SDSPAGE analysis; DNA-PCR, RT-PCR, qPCR; Preparation of tissue culture media; Tissue culture of tobacco leaf explants; Cell culture techniques; DNA-sequencing and DNA finger printing.

 

Assessment

Coursework 100%

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